Wrapper Arms Strategy to Construct Multicomponent Plasmids Using Three-Piece Ligation
نویسندگان
چکیده
منابع مشابه
Wrapper arms strategy to construct multicomponent plasmids using three-piece ligation.
1.Eastman Kodak Company. 1990. Kodak professional black-and-white films. Kodak Publication F-5. Rochester, NY. 2.Frohlich, M.W. and E.M. Meyerowitz. 1997. The search for homeotic gene homologs in basal angiosperms and gnetales: a potential new source of data on the evolutionary origin of flowers. Int. J. Plant Sci. 158:S131-S142. 3.Hamilton, B.A., M.J. Palazzolo and E.M. Meyerowitz. 1991. Rapid...
متن کاملUsing strong isomorphisms to construct game strategy spaces
When applied to the same game, probability theory and game theory can disagree on calculated values of the Fisher information, the log likelihood function, entropy gradients, the rank and Jacobian of variable transforms, and even the dimensionality and volume of the underlying probability parameter spaces. These differences arise as probability theory employs structure preserving isomorphic map...
متن کاملA wrapper to query DGIdb using R
Annotation and interpretation of DNA aberrations identified through next-generation sequencing is becoming an increasingly important task, especially in the context of data analysis pipelines for medical applications, where aberrations are associated with phenotypic and clinical features. A possible approach for annotation is to identify drugs as potential targets for aberrated genes or pathway...
متن کاملa paradigm shift away from method-wise teaching to strategy-wise teaching: an investigation of reconstructive strategy versus communicative strategy
چکیده: هدف اصلی این مطالعه ی توصیفی تحقیقی در حقیقت تلاشی پساروش-گرا به منظور رسیدن به نتیجه ای منطقی در انتخاب مناسبترین راهکار آموزشی بر گرفته از چارچوب راهبردی مطرح شده توسط والدمر مارتن بوده که به بهترین شکل سازگار و مناسب با سامانه ی آموزشی ایران باشد. از این رو، دو راهکار آموزشی، راهکار ارتباطی و راهکار بازساختی، برای تحقیق و بررسی انتخاب شدند. صریحاً اینکه، در راستای هدف اصلی این پژوهش، ر...
15 صفحه اولAn efficient stress-free strategy to displace stable bacterial plasmids.
A key stage in determining the phenotype(s) conferred by a plasmid is its displacement, or 'curing,' to create a plasmid-free strain. However, many plasmids are very stable, not only because they contain multiple replicons, but also because they can encode post-segregational killing systems that reduce the viability of plasmid-free segregants. We have developed an efficient curing strategy that...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00291bm04